Photo of Dr. Ryan Limbocker

Dr. Ryan Limbocker

Assistant Professor
ryan.limbocker@westpoint.edu

Biography


Ryan Limbocker is an Assistant Professor in the Department of Chemistry and Life Science and teaches general chemistry. Dr. Limbocker received the Goldwater Scholarship in 2014 and graduated with a B.S. in Chemistry from the University of Kansas with Highest Distinction, University Honors, and Departmental Honors. He attended graduate school at the University of Cambridge in the Centre for Misfolding Diseases funded by the St. John’s College Benefactors’ and Gates Cambridge Scholarships. Upon completion of his Ph.D. in Chemistry, he conducted a postdoctoral fellowship at Columbia University.

In addition to teaching and researching countermeasures and protein misfolding diseases, Dr. Limbocker is a passionate academic mentor. In particular, he actively seeks to help cadets consider, plan, and execute applications for technical, national, and international scholarships and prepare for medical school.

Ongoing Research Projects


The Limbocker research group focuses on understanding the etiology of protein misfolding diseases with emphasis on the pathological consequences of protein aggregation. By integrating in vitro biophysical methods with cellular models of toxicity, we aim to understand and target the principles that drive toxicity in neurodegenerative disorders ranging from Alzheimer’s and Parkinson’s diseases to Chronic Traumatic Encephalopathy. Our team also actively investigates the efficacy of small molecule countermeasures to protect Service members from acute traumatic events.

Limbocker Lab Members 2020

 

Front row (from left to right):  Cadet Natalie Block, Cadet Aidan Wright, Cadet Vani Verma, Cadet Mottura

Second row (from left to right):  Cadet Liam Sasser, Cadet Edward Custy, Cadet Joseph Garcia, Cadet Ryan Kreiser, Cadet Mark Nylund

Back row (from left to right):  Dr. Ryan Limbocker, MAJ Kathleen LeForte, CPT (P) Beau Nguyen, MAJ Jared Hollows

 

 

Publications & Presentations


Peer-Reviewed Publications

Priyanka Joshi, Michele Perni, Ryan Limbocker, Benedetta Mannini, Sam Casford, Sean Chia, Johnathan Labbadia, Christopher M. Dobson, Michele Vendruscolo. “Two human metabolites rescue a C. elegans model of Alzheimer's disease via a cytosolic unfolded protein response.” Communications Biology. 2021, 4, 843.

Ryan Limbocker, Roxine Staats, Sean Chia, Francesco S. Ruggeri, Benedetta Mannini, Catherine K. Xu, Michele Perni, Roberta Cascella, Alessandra Bigi, Liam R. Sasser, Natalie R. Block, Aidan K. Wright, Ryan P. Kreiser, Edward T. Custy, Georg Meisl, Silvia Errico, Johnny Habchi, Patrick Flagmeier, Tadas Kartanas, Jared E. Hollows, Lam T. Nguyen, Kathleen LeForte, Denise Barbut, Janet R. Kumita, Cristina Cecchi, Michael Zasloff, Tuomas P.J. Knowles, Christopher M. Dobson, Fabrizio Chiti, Michele Vendruscolo. “Squalamine and its derivatives modulate the aggregation of amyloid-β and α-synuclein and suppress the toxicity of their oligomers.” Frontiers in Neuroscience. 2021, 15, 680026.

Michele Perni, Annemieke van der Goot, Ryan Limbocker, Tjakko J. van Ham, Francesco A. Aprile, Catherine K. Xu, Patrick Flagmeier, Karen Thijssen, Pietro Sormanni, Giuliana Fusco, Serene W. Chen, Pavan K. Challa, Julius B. Kirkegaard, Romain F. Laine, Kai Yu Ma, Martin B.D. Müller, Tessa Sinnige, Janet R. Kumita, Samuel I.A. Cohen, Renée Seinstra, Gabriele S. Kaminski Schierle, Clemens F. Kaminski, Denise Barbut, Alfonso De Simone, Tuomas P.J. Knowles, Michael Zasloff, Ellen A.A. Nollen, Michele Vendruscolo, Christopher M. Dobson. “Comparative studies in the A30P and A53T α-synuclein C. elegans strains to investigate the molecular origins of Parkinson's disease.” Frontiers in Cellular and Developmental Biology. 2021, 9, 552549.

Gabriella T. Heller, Francesco A. Aprile, Thomas C.T. Michaels, Ryan Limbocker, Michele Perni, Francesco Simone Ruggeri, Benedetta Mannini, Thomas Löhr, Massimiliano Bonomi, Carlo Camilloni, Alfonso De Simone, Isabella C. Felli, Roberta Pierattelli, Tuomas P.J. Knowles, Christopher M. Dobson, Michele Vendruscolo. “Small-molecule sequestration of amyloid-β as a drug discovery strategy for Alzheimer’s disease.” Science advances. 2020, 6, 45, eaab5924.

Francesco A. Aprile, Pietro Sormanni, Marina Podpolny, Shianne Chhangur, Lisa-Maria Needham, Francesco S. Ruggeri, Michele Perni, Ryan Limbocker, Gabriella T. Heller, Tomas Sneideris, Tom Scheidt, Benedetta Mannini, Johnny Habchi, Steven F. Lee, Patricia C. Salinas, Tuomas P.J. Knowles, Christopher M. Dobson, Michele Vendruscolo. “Rational design of a conformation-specific antibody for the quantification of Ab oligomers.” Proceedings of the National Academy of Sciences U.S.A. 2020, 117, 13509-13518. 

Ryan Limbocker, Benedetta Mannini, Francesco S. Ruggeri, Roberta Cascella, Catherine K. Xu, Michele Perni, Sean Chia, Serene Chen, Johnny Habchi, Alessandra Bigi, Ryan P. Kreiser, Aidan K. Wright, J. Alex Albright, Tadas Kartanas, Janet R. Kumita, Nunilo Cremades, Michael Zasloff, Cristina Cecchi, Tuomas P.J. Knowles, Fabrizio Chiti, Michele Vendruscolo, Christopher M. Dobson. “Trodusquemine displaces protein misfolded oligomers from cell membranes and abrogates their cytotoxicity through a generic mechanism.” Communications Biology. 2020, 3, 485.

Ryan Limbocker, Benedetta Mannini, Rodrigo Cataldi, Shianne Chhangur, Aidan K. Wright, Ryan P. Kreiser, J. Alex Albright, Sean Chia, Johnny Habchi, Pietro Sormanni, Janet R. Kumita, Francesco S Ruggeri, Christopher M. Dobson, Fabrizio Chiti, Francesco A. Aprile, Michele Vendruscolo. “Rationally designed antibodies as research tools to study the structure–toxicity relationship of amyloid-b oligomers.” International Journal of Molecular Sciences. 2020, 21, 4542.

Ryan P. Kreiser, Aidan K. Wright, Natalie R. Block, Jared E. Hollows, Lam T. Nguyen, Kathleen LeForte, Benedetta Mannini, Michele Vendruscolo, Ryan Limbocker. “Therapeutic strategies to reduce the toxicity of misfolded protein oligomers.” International Journal of Molecular Sciences. 2020, 21, 8651.

Tatsuya Ikenoue, Francesco A. Aprile, Pietro Sormanni, Francesco S. Ruggeri, Michele Perni, Gabriella T. Heller, Christian P. Haas, Christoph Middel, Ryan Limbocker, Benedetta Mannini, Thomas C.T. Michaels, Tuomas P.J. Knowles, Christopher M. Dobson, Michele Vendruscolo. “A rationally designed bicyclic peptide remodels Aβ42 aggregation in vitro and reduces its toxicity in a worm model of Alzheimer’s disease.” Scientific Reports. 2020, 10, 15280.

Melissa Eslinger, Michael A. Washington, Carissa Pekny, Natalie Nepa, J. Kenneth Wickiser, Ryan Limbocker, G. Dennis Shanks. “Non-Pharmaceutical Interventions and Military Hygiene at the United States Military Academy between 1890 and 1910.” Military Medicine. 2020, usaa226.

Chi K. Nguyen, Daniel R. DeNeve, Lam T. Nguyen, Ryan Limbocker. “Impact of COVID-19 on general chemistry education at the United States Military Academy.” Journal of Chemical Education. 2020, 9, 2922-2927.

Ryan Limbocker, Sean Chia, Francesco S. Ruggeri, Michele Perni,  Roberta Cascella, Gabriella T. Heller, Georg Meisl, Benedetta Mannini, Johnny Habchi, Thomas C.T. Michaels, Pavan K. Challa, Minkoo Ahn, Samuel T. Casford, Nilumi Fernando, Catherine K. Xu, Nina D. Kloss, Samuel I.A. Cohen, Janet Kumita, Cristina Cecchi, Michael Zasloff, Sara Linse, Tuomas P.J. Knowles, Fabrizio Chiti, Michele Vendruscolo, Christopher M. Dobson. “Trodusquemine enhances Ab42 aggregation but suppresses its toxicity by displacing oligomers from cell membranes.” Nature Communications. 2019, 10, 225.

Michele Perni, Pavan K. Challa, Julius B. Kirkegaard, Ryan Limbocker, Mandy Koopman, Maarten C. Hardenberg, Pietro Sormanni, Thomas Muller, Kadi L. Saar, Lianne W.Y. Roode, Johnny Habchi, Giulia Vecchi, Nilumi Fernando, Samuel Casford, Ellen A.A. Nollen, Michele Vendruscolo, Christopher M. Dobson, Tuomas P.J. Knowles. “Massively parallel C. elegans tracking provides multi-dimensional fingerprints for phenotypic discovery.” Journal of Neuroscience Methods. 2018, 306, 57-69. 

Michele Perni, Patrick Flagmeier, Ryan Limbocker, Roberta Cascella, Francesco A. Aprile, Celine Galvagnion, Gabriella T. Heller, Georg Meisl, Serene W. Chen, Janet R. Kumita, Pavan K. Challa, Julius B. Kirkegaard, Samuel I.A. Cohen, Benedetta Mannini, Denise Barbut, Ellen A.A. Nollen, Cristina Cecchi, Nunilo Cremades, Tuomas P.J. Knowles, Fabrizio Chiti, Michael Zasloff, Michele Vendruscolo, Christopher M. Dobson. “Multistep inhibition of a-synuclein aggregation and toxicity in vitro and in vivo by trodusquemine.” ACS Chemical Biology. 2018, 13, 2308-2319.

Sam V. Kaplan, Ryan Limbocker, Beth Levant, Michael A. Johnson. “Regional differences in dopamine release in the R6/2 mouse Caudate Putamen.” Electroanalysis. 2018, 30, 1066–1072.

Michele Perni, Celine Galvagnion, Alexander Maltsev, Georg Meisl, Martin B.D. Muller, Pavan K. Challa, Julius B. Kirkegaard, Patrick Flagmeier, Samuel I.A. Cohen, Roberta Cascella, Serene W. Chen, Ryan Limbocker, Pietro Sormanni, Gabriella T. Heller, Francesco A. Aprile, Nunilo Cremades, Cristina Cecchi, Fabrizio Chiti, Ellen A.A. Nollen, Tuomas P.J. Knowles, Michele Vendruscolo, Ad Bax, Michael Zasloff, Christopher M. Dobson. “A natural product inhibits the initiation of a-synuclein aggregation and suppresses its toxicity.” Proceedings of the National Academy of Sciences U.S.A. 2017, 114, E1009–E1017.

Johnny Habchi, Sean Chia, Ryan Limbocker, Benedetta Mannini, Minkoo Ahn, Michele Perni, Oscar Hansson, Paulo Arosio, Janet R. Kumita, Pavan K. Challa, Samuel I.A. Cohen, Sara Linse, Christopher M. Dobson, Tuomas P.J. Knowles, Michele Vendruscolo. “Systematic development of small molecules to inhibit specific microscopic steps of Ab42 aggregation in Alzheimer’s disease.” Proceedings of the National Academy of Sciences U.S.A. 2017, 114, E200–E208.

Sam Kaplan, Ryan Limbocker, Rachel C. Gehringer, Jenny L. Divis, Gregory L. Osterhaus, Maxwell D. Newby, Michael J. Sofis, David P.  Jarmolowicz, Brooke D. Newman, Tiffany A. Matthews, Michael A. Johnson. “Impaired brain dopamine and serotonin release and uptake in Wistar rats following treatment with Carboplatin.” ACS Chemical Neuroscience. 2016, 7, 689-699. 

Meng Sun, Sam V. Kaplan, Rachel C. Gehringer, Ryan Limbocker, Michael A. Johnson. “Localized drug application and sub-second voltammetric dopamine release measurements in a brain slice perfusion device.” Analytical Chemistry. 2014, 86, 4151–4156.

Abstracts & Peer-Reviewed Conference Proceedings

Ryan Limbocker, Aidan K. Wright, Ryan P. Kreiser, Benedetta Mannini, Michele Vendruscolo. “Therapeutics against protein misfolded oligomers in neurodegenerative diseases.” Biophysical Journal. 2021, 3, 286a.

Ryan P. Kreiser, Aidan K. Wright, Lam T. Nguyen, Jared E. Hollows, Ryan Limbocker. “Cell membrane properties can mediate the toxicity of protein misfolded oligomers and folded toxins.” Biophysical Journal. 2021, 3, 308a.

Aidan K. Wright, Ryan P. Kreiser, Lam T. Nguyen, Jared E. Hollows, Ryan Limbocker. “Development of molecular countermeasures to modulate the populations and toxicity of Aβ42 oligomers.” Biophysical Journal. 2021, 3, 202a.

Priyanka Joshi, Michele Perni, Ryan Limbocker, Benedetta Mannini, Sam Casford, Sean Chia, John Labbadia, Christopher M. Dobson, Michele Vendruscolo. "Endogenous metabolites rescue a C. elegans model of Alzheimer's disease by triggering a heat shock response." FEBS Open Bio. Krakow, Poland. 2019, 9, 225.

Francesco Antonio Aprile, Pietro Sormanni, Michele Perni, Paolo Arosio, Ryan Limbocker, Shianne Chhangur, Benedetta Mannini, Sara Linse, Tuomas Knowles, Christopher M Dobson, Michele Vendruscolo. “Targeting amyloid formation using rationally designed antibodies.” Alzheimer’s & Dementia. London, UK. May 2018, 14, 7, P611. (Oral)

Ryan Limbocker, Benedetta Mannini, Sean Chia, Francesco S Ruggeri, Michele Perni, Roberta Cascella, Catherine Xu, Johnny Habchi, Janet R Kumita, Fabrizio Chiti, Tuomas PJ Knowles, Michele Vendruscolo, Christopher M Dobson. “Modulating amyloid-beta aggregation to reduce the toxicity of its oligomeric aggregates.” Biophysical Journal. San Francisco, CA, February 2018, 114, 3, P430a. (Poster)

Sean Chia, Johnny Habchi, Ryan Limbocker, Benedetta Mannini, Minkoo Ahn, Michele Perni, Oskar Hansson, Paolo Arosio, Janet R Kumita, Pavan Kumar Challa, Samuel IA Cohen, Sara Linse, Christopher M Dobson, Tuomas PJ Knowles, Michele Vendruscolo. “Systematic development of small molecules to inhibit specific microscopic steps of amyloid-beta42 aggregation in Alzheimer’s disease.” Biophysical Journal. San Francisco, CA, February 2018, 114, 3, 225a. (Poster)

Ryan Limbocker, Benedetta Mannini, Michele Perni, Sean Chia, Gabriella Heller, Francesco S Ruggeri, Johnny Habchi, Georg Meisl, Pavan K Challa, Michael Zasloff, Tuomas PJ Knowles, Michele Vendruscolo, Christopher M Dobson. “Attenuating the toxicity of amyloid-beta aggregation with specific species.” Biophysical Journal. New Orleans, LA, February 2017, 112, 3, 494a. (Oral and poster)